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2. Cell Preparation and Loading (a) Rinse cultures with 5 ml of TBSS (volumes indicated here are for 10-cm dishes) and remove liquid. (b) Add 4 ml trypsin-EDTA. Incubate at 37 C for 5 6 min. (c) Add 2 ml BCS. Pipette resulting cell suspension up and down and gently rinse remaining cells off plate. Do not try to get all the cells off, just the easily detachable ones. (d) Transfer suspension to 15- or 50-ml conical tube and centrifuge at 350 g for 5 min. (e) Remove supernate and resuspend cell pellet in 10 ml serum-free DMEM-LG. (f) Centrifuge as in (d) above. (g) Remove supernate and resuspend pellet in 5 ml serum-free medium. (h) Determine the number of cells with a hemocytometer or electronic cell counter. (i) Centrifuge and resuspend pellet in serum-free medium at 5 106 cell/ml. (j) Transfer cell suspension to a sterile 12 75-mm polystyrene tube. (k) Add an appropriate number of bronectin-coated cubes to the cell suspension. (l) Draw off air from the tube as above (step (1)(i)), but with a 20-ml syringe. Flick tube to eliminate bubbles. (m) Replace cap with a new one. (n) Place tube in a 37 C incubator with cap loose (to allow for CO2 equilibration) for at least 2 h.

There is a huge need to study how sociocultural forces contribute to the ways that the psychiatric indicators and coping style are expressed and the nuances of the patient s communication style These factors may also affect access to and personal value attached to different resources (eg, social versus.

Hollingworth, L S (1916) Social devices for impelling women to bear children American Journal of Sociology, 22, 19 29 Horner, M S (1970) Femininity and successful achievement: A basic inconsistency In J M Bardwick, E Douvan, M S Horner, & D Gutman (Eds), Feminine personality and con ict (pp 45 74) Belmont, CA: Brooks/Cole Hurtado, A (1989) Relating to privilege: Seduction and rejection in the subordination of White women and women of color Signs: Journal of Women in Culture and Society, 14, 833 855 Hyde, J S, & Linn, M (1988) Sex differences in verbal ability: A meta-analysis Psychological Bulletin, 104, 53 69 Johnson, B T, & Eagly, A H (2000) Quantitative synthesis of social psychological research In H T Reis & C Judd (Eds), Handbook of research methods in social and personality psychology (pp 496 528) New York: Cambridge University Press Johnson, N G.

, Roberts, M C, & Worell, J (Eds) (1999) Beyond appearance: A new look at adolescent girls Washington, DC: American Psychological Association Jordan, J V, Kaplan, A G, Miller, J B, Stiver, I P, & Surrey, J (1991) Women s growth in connection New York: Guilford Press Kaschak, E (1992) Engendered lives New York: HarperCollins Katz, P (1991) Women, psychology, and social issues research Psychology of Women Quarterly, 15, 665 675 Kessler, S, & McKenna, S (1978) Gender: An ethnomethodological approach New York: Wiley Kimmel, E B (1974) The status of women in the southeast: A case study American Psychologist, 29, 519 540 Kimmel, E B, & Crawford, M (Eds) (2000) Innovations in feminist psychological research New York: Cambridge University Press Kitzinger, C (1994) Should psychologists study sex differences Feminism and Psychology, 4, 501 546 Koss, M P (1993).

Rape: Scope, impact, interventions, and public policy responses American Psychologist, 48, 1062 1069 Lakoff, R (1975) Language and woman s place New York: Harper & Row Lamb, S (1996) The trouble with blame: Victims, perpetrators, and responsibility Cambridge, MA: Harvard University Press Lamb, S (1999) New versions of victims: Feminists struggle with the concept New York: New York University Press Landrine, H (1995) Bringing cultural diversity to feminist psychology: Theory, research, and practice Washington, DC: American Psychological Association Lerman, H (1976) What happens in feminist therapy In S Cox (Ed), Female psychology: The emerging self Chicago: Science Research Associates Lerner, H G (1988) Women in therapy New York: Harper & Row Lykes, M B (1989) Dialogue with Guatemalan Indian women: Critical perspectives on constructing collaborative research In.

I reserve the term user-oriented application for an Excel application that can be used by someone with minimal training. These applications produce useful results even for users who know virtually nothing about Excel. The Loan Amortization Wizard discussed in this chapter qualifies as a user-oriented application because it s designed in such a way that the end user doesn t need to know the intimate details of Excel to use it. Replying to a few simple prompts produces a useful and flexible worksheet complete with formulas.

(1) L 1 (b i / Y )